Genetic engineering: Human insulin
نویسندگان
چکیده
منابع مشابه
Genetic engineering in mice: impact on insulin signalling and action.
The expression of a number of genes encoding key players in insulin signalling and action, including insulin, insulin receptor (IR), downstream signalling molecules such as insulin receptor substrate-1 (IRS-1) and IRS-2, glucose transporters (GLUT4, GLUT2) and important metabolic enzymes such as glucokinase, has now been altered in transgenic or knockout mice. Such mice presented with phenotype...
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BglII 5’...A_GATCT...3’ (5’overhang) BamHI 5’...G_GATCC...3’ (5’overhang); notice: MboI and Sau3AI = 5’..._GATC...3’ EcoRI 5’...G_AATTC...3’ (5’ overhang) HpaII 5’...C_CGG...3’ (5’ overhang); notice: CpG methylation blocked HindIII 5’...A_AGCTT...3’ (5’ overhang) KpnI 5’...GGTAC_C...3’ (3’ overhang) NcoI 5’...C_CATGG...3’ (5’ overhang); notice: Kozak sequence (RCC)ACCATGG NotI 5’...GC_GGCCGC......
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ژورنال
عنوان ژورنال: Nature
سال: 1982
ISSN: 0028-0836,1476-4687
DOI: 10.1038/300100c0